Postnova Analytics. This white paper demonstrates how Postnova Analytics’ novel Electrical and Asymmetrical Field Flow Fractionation (EAF4) technology can be used to separate a wide variety of nano-sized materials while simultaneously determining surface charge. Drawing upon this proprietary technology, the Postnova Analytics EAF2000 is the only field-proven system that combines the principle of Electrical and Asymmetrical Field Flow Fractionation in a single platform. In an EAF2000 system, Electrical and Cross Flow Fields are applied together across the FFF channel enabling separation by size and simultaneous measurement of particle charge based on electrophoretic mobility. Combining these two powerful separation techniques in a single platform opens the door to characterizing complex samples that have proven impossible using other techniques. With installed systems in leading academic and government research institutions as well as major pharmaceutical laboratories in Asia, Europe and North America - Postnova Analytics' pioneering EAF2000 system is proven to separate and characterize complex proteins, antibodies and viruses as well as charged nanoparticles, colloids and polymers.